Alhamdulillah, the lab work has really started. Tonight we are going to test the autoclave for the first time. Hj Naza has filled up the flasks with M39 during the day. The media need to be sterilize before flasking work can begin later. The autoclave was set to 120 degree Celcius, 15 psi and flasks were sterilized for 15 minutes. In reality, it takes the autoclave 15-20 minutes to warmup, and then maintain for 15 minutes at 15 psi before it dries out and complete the cycle. Overall, it takes about less than 1 hour to complete a single process. Hmm.. i guess the autoclave has done a good job but we need to further refine our technique.
 |
| 4 crates of flasks - 200 flasks in total |
 |
| Flask filled with M39 media |
 |
| The cap if further tighten up with rubber band |
|
 |
| 16 flasks per level |
 |
| A total of 57 flasks can be put inside at one time |
 |
| This is how it looks like after nearly 1 hour of 'cooking' |
 |
| The media is still boiling after being taken out from autoclave |
 |
Flasks are arranged properly with care
|
