Autoclave Works Started - Preparing Culture Medium No 39 (M39)

Alhamdulillah, the lab work has really started. Tonight we are going to test the autoclave for the first time. Hj Naza has filled up the flasks with M39 during the day. The media need to be sterilize before flasking work can begin later. The autoclave was set to 120 degree Celcius, 15 psi and flasks were sterilized for 15 minutes. In reality, it takes the autoclave 15-20 minutes to warmup, and then maintain for 15 minutes at 15 psi before it dries out and complete the cycle. Overall, it takes about less than 1 hour to complete a single process. Hmm.. i guess the autoclave has done a good job but we need to further refine our technique.  

4 crates of flasks - 200 flasks in total

Flask filled with M39 media

The cap if further tighten up with rubber band

16 flasks per level

A total of 57 flasks can be put inside at one time

This is how it looks like after nearly 1 hour of 'cooking'

The media is still boiling after being taken out from autoclave

Flasks are arranged properly with care

Finally It was Delivered!

The LAF and autoclave are here already! Mikail sent them both on Saturday around 2.30 pm. but they need to calibrate the LAF next week on Saturday.

Laminar Air Flow - RM9k

Autoclave - RM14k

Hellooo.. I Am Still Waiting!

Aargghh.. Mikhail said he can only give the quotation of lab equipment today. The LAF will only be ready by next week. Seems my schedule has to be pushed forward...

In business, delay means loss! If the operation has started in March, for example, the company could have generated revenue as projected. Businessman should be very concerned on 'delay' as this will affect a lot of things. Now due to this, the company has incurred loss for one month!